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Axis-Shield Diagnostics polymorphprep™ density gradient media
Polymorphprep™ Density Gradient Media, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+gradient/polymorphprep+density+gradient/pm37311354-46-11-15
Average 90 stars, based on 1 article reviews
polymorphprep™ density gradient media - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: NLRP1-dependent activation of Gasdermin D in neutrophils controls cutaneous leishmaniasis
Article Snippet: Reconstituted blood was spiked with 5x10 6 / mL Leishmania mexicana (MYNC/BZ/62/M379 strain) procyclic promastigotes passaged the day before sand fly infection in Schneider’s media (Gibco) supplemented with 20% fetal bovine serum (Thermo-fisher Scientific) and 1% penicillin/streptomycin (Gibco) and kept at 26°C. .. Neutrophils were isolated by centrifugation over a polymorphprep (Axis-Shield) gradient as previously described [ ]. .. Purity of neutrophils (>95%) was assessed by cytospin and Diff-quick staining (RAL Diagnostics).

Article Title: Exogenous Serpin B1 restricts Immune complex-mediated NET formation via inhibition of a chymotrypsin-like protease and enhances microbial phagocytosis.
Article Snippet: .. Neutrophils were isolated from healthy subjects with Polymorphprep (Axis-Shield, Dundee, UK) density gradient as described previously 66. ..

Article Title: NLRP1-dependent activation of Gasdermin D in neutrophils controls cutaneous leishmaniasis.
Article Snippet: .. Neutrophils were isolated by centrifugation over a polymorphprep (Axis-Shield) gradient as previously described [89]. .. Purity of neutrophils (>95%) was assessed by cytospin and Diff-quick staining (RAL Diagnostics).

Centrifugation:

Article Title: NLRP1-dependent activation of Gasdermin D in neutrophils controls cutaneous leishmaniasis
Article Snippet: Reconstituted blood was spiked with 5x10 6 / mL Leishmania mexicana (MYNC/BZ/62/M379 strain) procyclic promastigotes passaged the day before sand fly infection in Schneider’s media (Gibco) supplemented with 20% fetal bovine serum (Thermo-fisher Scientific) and 1% penicillin/streptomycin (Gibco) and kept at 26°C. .. Neutrophils were isolated by centrifugation over a polymorphprep (Axis-Shield) gradient as previously described [ ]. .. Purity of neutrophils (>95%) was assessed by cytospin and Diff-quick staining (RAL Diagnostics).

Article Title: NLRP1-dependent activation of Gasdermin D in neutrophils controls cutaneous leishmaniasis.
Article Snippet: .. Neutrophils were isolated by centrifugation over a polymorphprep (Axis-Shield) gradient as previously described [89]. .. Purity of neutrophils (>95%) was assessed by cytospin and Diff-quick staining (RAL Diagnostics).

other:

Article Title: Knockdown of versican 1 in lung fibroblasts aggravates Lipopolysaccharide-induced acute inflammation through up-regulation of the SP1-Toll-like Receptor 2-NF-κB Axis: a potential barrier to promising Versican-targeted therapy.
Article Snippet: Objective: Versican participates in various pathological processes like inflammation and fibrosis and is a potential therapeutic target for inflammatory diseases.. Versican 1 (V1) has increased expression in inflammatory diseases, but its role is unclear.. We explored the effects of V1 on acute lung inflammation to determine whether targeting V1 had therapeutic potential.

Article Title: Long noncoding RNA SNHG16 regulates TLR4-mediated autophagy and NETosis formation in alveolar hemorrhage associated with systemic lupus erythematosus.
Article Snippet: PB neutrophils (PBNs) were obtained by PolymorphprepTM (AXIS-SHIELD PoC AS, Oslo, Norway) gradient separation for the granulocyte fraction with the removal of RBCs by hypotonic lysis.

Article Title: Mycobacterium tuberculosis PE/PPE proteins enhance the production of reactive oxygen species and formation of neutrophil extracellular traps
Article Snippet: Human neutrophils were isolated and purified from fresh heparinized venous blood by density gradient centrifugation at 480xg using PolymorphprepTM (Axis-Shield, PoC) as previously described ( ).

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: Neutrophils were isolated from peripheral blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: In panel two, blood from the normal, OLK and OSCC stages was collected from the angular vein of rats, and neutrophils were isolated from blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.



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